抗氧化剂对犬门腔分流术肝缺血损害的保护作用

PROTECTIVE EFFECTS OF ANTIOXIDANTS ON HEPATIC ISCHEMIC DAMAGE AFTER PORTACAVAL SHUNT IN DOGS

目的:探讨脂质过氧化反应在门腔分流术后肝脏缺血损害中的作用及抗氧化剂川芎嗪,辅酶Q10的保护作用。方法:将24条成年杂种犬实施门腔静脉分流术后随机分为三组:单独门腔分流组(PCS组),门腔分流加川芎嗪组(PCS+TMP组),门腔分流加川芎嗪加辅酶Q10组(PCS+TMP+CO—Q10组),连续术后8周追踪检测血中脂质过氧化产物丙二醛(MDA),超氧化物歧化酶(SOD),谷丙转氨酶(ALT),谷草转氨酶(AST),硷性磷酸酶(AKP),并取肝组织作光镜及电镜观察。结果:PCS组术后MDA、ALT、AST、AKP持续升高,SOD下降,肝脏结构损害明显。PCS+TMP组和PCS+TMP+CO-Q10组明显优于PCS组(P<O.05或P<O.01)。同时,PCS+TMP+CO-Q10组亦优于PCS+TMP组(P<0.05)。结论:体内过度激活的脂质过氧化反应是分流术后肝缺血损害的重要因素之一,联合应用抗氧化剂可增强对肝缺血损害的保护作用。

Objective: To study the role of lipid peroxide reaction in hepatic ischemia damage and the protective effects of antioxidants, ligustrazine and coenzyme Q10(CO -Q10). Method: Portacaval shunt was performed in 24 dogs and they were divided randomly into three groups, i.e. control group, ligustrazine group and combined group ( ligustrazine + Co - Q10 ). After the operation, the malondialhyde (MDA). superoxide dismutase (SOD), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) in blood of these dogs were detected continously for 8 weeks. The canine liver tissues were observed under light and electronic microscopes. Result: The level of MDA, ALT, AST and AKP increased continously, SOD decreased and liver tissues were damaged obviously in control group. Compared with control group, the changes of both treated groups (P<0. 01 或 P < 0. 05 ). Results of combined group were better than those of ligustrazine group (P<0. 05). Conclusion: The over- activated lipid peroxide reaction may be one of the important factors which induce hepatic ischemia damage after portacaval shunt. Combined use of ligustrazine and coenzyme Q10 can enhance the protective effects on hepatic ischemia damage.