不同牙龈卟啉单胞菌菌株对人牙周膜细胞MMP-3和MMP-9表达的影响

Matrix metalloproteinase 3 and 9 regulations of human periodontal ligment cells stimulated by Porphyromonas gingivalis with different strains

【目的】比较3种牙龈卟啉单胞菌菌株P.gingivalis W83、P.gingivalis ATCC33277、P.gingivalis FDC381刺激人牙周膜细胞表达基质金属蛋白酶(matrix metalloproteinases,MMPs)MMP-3、MMP-9水平的差异。【方法】厌氧培养细菌P.gingivalis W83、P.gingivalis ATCC33277和P.gingivalis FDC381,原代培养人牙周膜细胞,3种菌株分别与人牙周膜细胞混合培养5 min、30 min、60 min、120 min、24 h、48 h,采用ELISA法测定上清液中MMP-3和MMP-9的浓度。【结果】在各时间点,受细菌刺激的人牙周膜细胞表达MMP-3和MMP-9的浓度均高于未受细菌刺激组(P<0.01)。P.gingivalis W83刺激人牙周膜细胞表达的MMP-3、MMP-9浓度明显高于P.gingivalis ATCC33277和P.gingivalis FDC381,经方差分析,差异具有显著性(P<0.01)。【结论】高毒力株P.gingivalis W83可以通过细菌-细胞间相互作用导致局部牙周组织中MMP-3、MMP-9高表达,造成牙周组织破坏。

[Objective] To investigate the pathogenicity of matrix metalloproteinase 3, 9 （MMP-3, MMP-9） regulations of human periodontal ligment cells （hPDLCs） challenged by different P. gingivalis strains. [Methods] Human periodontal ligment cells were cultured using explant culture technique. P. gingivalis W83, P. gingivalis ATCC33277 and P. gingivalis FDC381 were assessed for their inductions of MMP-3, MMP-9 expression in human periodontal ligment ceils. MMP-3, MMP-9 protein levels in culture supernatant were determined by ELISA at the different time intervals （5 min, 30 min, 60 min, 120 min, 24 h, 48 h） following continuous co-culture of bacteria with hPDLCs. [Results] MMP-3 and MMP-9 protein levels produced by hPDLCs co-culture with P. gingivalis W83, P. gingivalis ATCC33277 and P. gingivalis FDC381 were significantly stronger than unsimulated group （P 〈 0.01）. The concentrations of MMP-3 and MMP-9 produced by hPDLCs co-culture with the P. gingivalis W83 were more than P. gingivalis ATCC33277 and P. gingivalis FDC381 （P 〈 0.01）. [Conclusion] P. gingivalis W83 has stronger pathogenicity for periodontal disease, which probably depending on the bacteria-cell interaction producing much matrix metalloproteinase in local.