细胞周期蛋白D1基因G870A多态性与结直肠癌易感性的系统评价

Cyclin D1 G870A polymorphism and the risk of colorectal cancer： a systematic review

目的综合评价细胞周期蛋白D1（CyclinD1）基因G870A多态性与结直肠癌易感性的关系。方法通过CNKI、PubMed、EMCC等数据库检索文献，获得有关CyclinD，基因G870A多态性与结直肠癌危险性关系的研究结果，并通过Meta分析进行系统评价。所有文献均采用病例对照研究或者巢式病例对照研究，以OR值为效应指标，基因型在对照群体中的分布均符合Hardy—Weinberg遗传平衡定律，对文献进行评价筛选、异质性检验。本次Meta分析共纳入23项研究，累计病例6344例，对照9018例，利用RevMan5．0对各研究原始结果进行统计处理，对突变纯合子AA和杂合子GA基因型与纯合基因型GG进行比较，并计算合并OR值及其95％可信区间（cI）。结果AA和GA与GG基因型在病例组与健康对照组之间差异有统计学意义，OR=1．10（95％CI：1．01～1．19，P：0．02）；按不同人群进行分层分析，亚洲人群OR=1．11（95％CI：0．98～1．26，P=0．11），美洲人群OR=1．13（95％CI：0．97～1．32，P=0．12），欧洲人群OR=1．06（95％CI：0．89—1．25，P=0．52），大洋洲人群OR=1．05（95％C1：0．80～1．38，P：0．73）；按不同对照进行分组分析，医院基础OR=1．07（95％CI：0．95～1．20，P=0．28），人群基础OR：1．13（95％CI：1．01～1．26，P=0．04）。结论CyclinD1基因G870A多态性与结直肠癌易感性总体分析在统计学上具有相关性，按不同人群进行分组分析，都不支持具有相关性；按不同对照进行分组分析，以医院基础不具有相关性，以人群为基础具有相关性。

Objective To evaluate the association between Cyclin D1 Gg70A polymorphism and risk of colorectal cancer. Methods Extensive searches of relevant studies on datebase like PubMed, EMCC and CNKI were performed. Case control studies involving unrelated subjects and genotype frequencies in control group consist- ent with Hardy-Weinberg equilibrium were included for the meta-analysis. Twenty-three case-control studies with 6 344 cases and 9 018 controls were analyzed by the fixed-effect or random-effect meta-analysis method. The metaanalysis was applied with RevMan 5.0 software for heterogeneity test. AA and GA were compared with those with GG genotype. Pooled OR value and 95% confidence interval （CI） were calculated. Results There were statistical differences between AA ＆ GA and GG. The pooled OR value was 1.10 （95% CI：I. 01-1.19, P =0. 02）. The values were analyzed hierarchically according to different populations. The pooled OR value of Asian was 1.11 （95% （21： 0.98-1.26, P =0.11）. The pooled OR value of American was 1.13（95%CI：0.97-1.32, P=0.12）. The pooled OR value of European was 1.06（95%CI：0.89-1.25, P =0.52）. The pooled OR value of Oceanian was 1.05（95%CI： 0. 80-1.38, P = 0. 73）. The values were analyzed hierarchically according to the comparison basis. The pooled OR value of hospital-based was 1.07（95% CI：O. 95-1.20, P =0.28）. The pooled OR value of population-based was 1.13 （95% CI：I. 01-1.26, P =0. 04）. Conclusion The Cyclin D1 G870A pelymorphism is correlated with the suscepti- bility of colorectal cancer risk at the aggregate level analysis. Analysis by different methods ： according to different populations, every group don＇t support the correlation. According to comparison basis, there has no correlation in hospital-based group, but there has correlation in population-based group.