实验性脑缺血再灌注大鼠外周血CINC的变化

The changes of cytokin-induced neutrophil chemoattractant (CINC) in blood of experimental cerebral ischemia reperfusion with rats

目的:为了探讨脑缺血再灌注外周血细胞因子介导的粒细胞趋化因子(CINC)水平的变化,采用动物实验方法对实验性脑缺血再灌注不同时间内外周血CINC和髓质过氧化物酶(MPO)活性的变化进行了观察。方法:选用改良线栓法复制Sprague-Dawley(SD)大鼠脑缺血再灌注实验模型。对不同时间内的血清MPO活性及CINC水平进行测定。结果:单纯缺血组MPO活性增高始于缺血6h并于缺血72h时达高峰,为0.15±0.01U/ml;CINC水平增高始于缺血1h,缺血6h时达高峰,为0.05±0.05ng/ml,以后逐渐下降并于缺血48h后恢复正常。缺血1小时再灌注组MPO活性增高始于再灌.注1h并于再灌注24h时达高峰,为0.42±0.12U/ml;CINC水平增高始于再灌注1h,再灌注3h时达高峰,为0.65±0.03ng/ml,以后逐渐下降并于再灌注24h后恢复正常。结论:实验性脑缺血再灌注外周血CINC有表达增高改变,其作用过程可能与白细胞激活过程有关,提示CINC增高可能在脑缺血再灌注损伤中起一定作用。

Objective: The purpose of study was to assess the changes rule of cytokin-induced neutrophil chemoattractant (CINC). Methods; In different time, the concentrations of CINC and the activities of myeloperioxidase (MPO) were observed in experimental cerebral ischemia reperfusion model with Sprague-Dawley (SD) rats made by modified thread embolism method. Results: In ischemia group, the peaks of MPO activity and CINC conentration were 24 hour and 6 hour respectively after ischemia, which initially increased at 1 hour, In ischemia reperfusion group, the peaks of MPO activity and CINC conentration were 72 hour and 6 hour respectively after ischemia reperfusion, which initially increased at 6 hour and Ihour down to normal at 24hour respectively. Conclusion: The rise of CINC was an effect of activiting WBC after the ischemia or ischemia reperfusion and indicated that the change was involving in the damage of ischemia reperfusion.