老化对小鼠激活淋巴细胞分泌白细胞介素3及其mRNA表达的影响

Effect of Aging on Interleukin 3 and Its mRNA Levels Expression in Mouse Lymphocytes

本实验测定老化对小鼠激活淋巴细胞分泌白细胞介素3(Interleukin 3,IL-3)和其基因表达的影响。结果5月龄与37月龄小鼠相比较,后者IL-3活性下降79.3％,IL-3mRNA水平下降74％,二者IL-3mRNA水平高峰均位于Con A刺激培养后20h。Northern印迹分析证明,4月龄与27月龄小鼠IL-3mRNA的位置均无移动。用ConA刺激4组不同年龄小鼠的脾淋巴细胞,比较其IL-2和IL-3活性及其mRNA水平,证明二淋巴因子随着年龄增加而逐渐下降的水平具有平行性。

In this study, we determined IL-3 and its mRNA levels in T cells from C57BL/6J mice of different age groups (5, 12, 23 and 37 months). Spleen lymphocytes were stimulated with ConA and IL-3 activity was assayed by their ability to support the growth of an IL-3 dependent cell line, FDCP-1. IL-3 mRNA was determined by cytoplasmic dot-blot hybridization. mRNA reached maximum levels after 20 h of incubation with ConA in all 4 groups. The IL-3 activity and mRNA level in the 37 month age group showed 79.3% and 74% decreases respectively in comparison with the 5 month age group. The IL-2 and IL-3 mRNA levels also showed a parallel decline with age.