家兔主动脉平滑肌细胞条件培养基抑制血管平滑肌细胞增殖

The Conditioned Medium from Rabbit Aortic Smooth Muscle Cells Inhibited Proliferation of Vascular cular Smooth Muscle Cell

为探讨血管平滑肌细胞自分泌和旁分泌对血管平滑肌细胞增殖的抑制作用 ,采用家兔主动脉贴块培养法培养血管平滑肌细胞 ,并制备平滑肌细胞条件培养基。采用孔径 10 0kDa和 10kDa的Millipore滤膜 ,将平滑肌细胞条件培养基分部。平滑肌细胞增殖测定采用宝灵曼试剂盒XTT法。结果提示 ,家兔血管平滑肌细胞条件培养基抑制血管平滑肌细胞的增殖 ,浓度 5 0 %的平滑肌细胞条件培养基抑制血管平滑肌细胞的增殖率高达 45 % ,且该生长抑制活性物质具有剂量依赖、加热 5 6℃ 30min稳定和分子质量小于 10kDa的特点。

Aim To ascertain whether vascular smooth muscle cells inhibit vascular smooth muscle cell proliferation by autocrine or paracrine of growth inhibitors. Methods Conditioned medium from rabbit aortic smooth muscle cell was used in this study. The conditioned medium was filtered by cut off M.W. 100 kDa or 10 kDa (Millipore). Smooth muscle cell proliferation was assayed by the measurement of XTT (Boehringer Mannhein). Results Rabbit aortic smooth muscle cell conditioned medium inhibited smooth muscle cell proliferation, 50% smooth muscle cell conditioned medium achieved 45% inhibitory rate of smooth muscle cell proliferation. This inhibition activity substance is dose dependent, 56℃, 30 min heat stable and M.W.less than 10 kDa. Conclusions Rabbit aortic smooth muscle cell could inhibit smooth muscle cell proliferation by autocrine or paracrine growth inhibitors.