5,6-二苯基哒嗪-3-酮衍生物对胞浆微管复合物作用的免疫荧光观察

Immunofluorescent Studies on Cytoplasmic Microtubule Complex (CMTC) of Vero Cells Treated with 5,6-Diphenylpyridazin-3-one Derivatives

本实验应用管蛋白间接免疫荧光法,观察5,6-二苯基哒嗪-3-酮(5,6-diphenylpyrida-zin-3-one,DPP)的6个衍生物对胞浆微管复合物(cytoplasmic microtubule complex,CMTC)的作用。结果表明,这6个衍生物对胞浆微管复合物的作用活性强弱不同,且具有剂量依赖性。其中PD033对胞浆微管复合物的作用很弱,PD032除对体外微管聚合有较强作用外,对胞浆微管复合物也有较强作用,并且在部分细胞胞浆内形成短束状异常结构,其分布特征与紫杉醇(taxol)引起的胞浆内自由微管的分布特征有某些相似之处。

Effects of six derivatives of 5,6-diphenyl-pyridazin-3-one (DPP) on CMTC of Vero cells were studied by means of the tubulin indirect immunofluorescence technique. Results showed that the effects were different with different derivatives and dose-depsndent. Although PD033 had been known to be potent in inhibiting microtubule assembly in vitro, it showed little effect on disturbing CMTC in vivo. Besides having the effects mentioned above, PD032 also induced the formation of free microtubules similar to those induced by taxol.