山莨菪碱抑制佛波酯和A_(23187)刺激的巨噬细胞的前列腺素合成

Inhibition of Phorbol Ester and A_(23187)- Stimulated Prostaglandins Synthesis in Macrophages by Anisodamine

山莨菪碱抑制巨噬细胞受佛波酯PMA和钙离子载体A_(23187)刺激时的前列腺素合成,经山茛菪碱预先温育,细胞的前列腺素合成也明显减少。山莨菪碱对外源性花生四烯酸刺激的前列腺素合成无抑制作用。结果提示山莨菪碱作用发生在磷脂酶A_2水平。

The effect of anisodamine on arachidonic acid metabolism in macrophages was studied . The results indicated that the synthesis of PGI2 , TXA2 and PGF2α stimulated by phorbol- 12-myristate- 13-acetate (PMA)and calcium ionophore A23187 was dose and time - dependency inhibited by anisodamine, and preincubation of the cells with anisodamine significantly decreased subsequent PMA and A23187 -stimulated synthesis of prostaglandins . Anisodamine failed to inhibit exogenous arachidonic acid - stimulated synthesis of prostaglandins. It is suggested that the site of action of anisodamine could be the phospholipase A2 reaction .