摘要
目的建立方格星虫多糖中糖醛酸的含量测定方法。方法采用咔唑-硫酸比色法,以半乳糖醛酸为对照品,在最大吸收波长525nm处测定吸光度值(OD)。结果半乳糖醛酸的线性范围为2.75～27.5μg/ml(R2=0.9995),方法重现性实验RSD为1.22%(n=6),平均回收率为103.5%,RSD为1.9%。三批方格星虫多糖中糖醛酸含量分别为23.68%,22.87%和25.08%。结论该方法简便、灵敏、重复性好,可以作为方格星虫多糖中糖醛酸的质量控制方法。
Objective To determine the uronic acid in polysaccharides from Sipunculus nudus Linnaeu. Methods The contents of uronic acid in polysaccharide was studied by carbazole and sulphuric acid method with galaeturonie acid as standard , the absorb- ency on 525 nm was measured. Results The linear range of galacturonic acid was 2.75 - 27.5 μg/ml（ R2 = 0. 9995 ）. The repitition RSD of uronic acid was 1.22% （n =6） and the average recovery was 103. 5% （RSD = 1. 9% ）. The contents of uronic acid in polysaccarides of three batchs were 23.68% ,22.87% and 25.05% ,respectively. Conclusion The established method is simple, sensitive and accurate which can be applied to control the content of uronic acid in polysaecarides from Sipunculus nudus Linnaeu.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第5期1100-1101,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家科技重大专项新药创制项目(No.2008ZXJ09004-027)
关键词
方格星虫
糖醛酸
多糖
咔唑-硫酸比色法
Sipunculus nudus
Uronic acid
Polysaccharide
Carbazole and sulphuric acid method
