人血浆抗利尿激素放射免疫分析法

Radioim m unoassay of Arginine Vasopressin in Hum an Plasma

用抗利尿激素抗血清建立人血浆抗利尿激素放射免疫分析法,用Iodogen法制备标记化合物^(125)I-sAVP,比放射强度达1369mCi/mg,-25℃可储存1个月。标准曲线灵敏度为0.15pg/管,血浆AVP最小可测量为0.6pg/ml,测定范围为0.6～11.0pg/ml。用丙酮—石油醚法提取血浆AVP,回收率为75.6％,批内和批间变异系数分别为9.8％和16.8％,提示提取是放射免疫法测定人血浆AVP的必须步骤。本法所得结果能充分反映正常人血浆AVP的生理波动。

A sensitive radioimmunoassay for human plasma arginine vasopressin was established. ^(125)I-sAVP (1369mCi/mg)was first produced by the Iodogen method and could be u for up to one month in -25℃ storage. The sensitivity was 0.15 pg/tube. The sensitivity of the assay was 0.60 pg/ml, with a range of 0.6～11.0 pg/ml. The acetone-ethnol petroleum method was used to extract vasopressin from plasma samples, with a recovery rate of 75.6％. The intra-and inter-assay coefficients of variation were 9.8％ and 16.8％ respectively. Plasma vasopressin extraction was proved to be indispensible to the assay by virtue of its ability to minimize plasma protein interference. The physiological fluctuation of human plasma vasopressin with and without water intake was shown to be significant between normal adults and patients with neurogeuic diabetes insipidus.