BN大鼠致敏动物模型研究

Study on animal model of testing food allergenicity in BN rat

目的建立经腹腔注射途经给予造模致敏原的BN大鼠致敏动物模型。方法分别在实验第1、5和10天,经腹腔注射给予不同性别(雌性和雄性)和不同周龄(4周龄和8周龄)的BN大鼠,不同剂量(1.00、0.10和0.01mg)的造模致敏原———卵清蛋白(OVA),观察35天。分别于第28、35天内眦取血分离血清,用ELISA方法检测血清中OVA特异性IgE。结果与阴性对照组相比,雌性8周龄BN大鼠中、高剂量组第28、35天血清中OVA特异性IgE浓度显著升高(P<0.05);雄性8周龄BN大鼠低、中剂量组血清中OVA特异性IgE浓度在第28天显著升高(P<0.05),高剂量组血清中OVA特异性IgE浓度在第35天显著升高(P<0.05);雄性4周龄BN大鼠,高剂量组血清中OVA特异性IgE浓度在第35天显著升高(P<0.05)。结论经腹腔注射途径给予不同性别和周龄的BN大鼠不同剂量的OVA,雌性大鼠比雄性大鼠更敏感;8周龄大鼠比4周龄大鼠更敏感;0.10mg或1.00mg的致敏剂量较适合。因此,选用雌性8周龄BN大鼠,经腹腔注射给予0.10mg或1.00mgOVA,35天后即可建立比较理想的BN大鼠致敏动物模型。

Objective To establish a BN rat model for testing food allergenicity. Methods BN rats of both sex and aged 4 and 8 weeks were exposed to different doses of ovalbumin （OVA） （1.00mg, 0. 10rag and 0.01mg） by intraperitoneal injection on the 1st, 5th and 10th day of the study and observed for 35 days. Blood samples were taken on the 28th and 35th day of the study from orbital plexus and serum specific lgE （OVA sIgE） were determined by ELISA. Results Compared with the control group, the concentrations of OVA sIgE of 8-week female BN rats on the 28th and 35th day were significantly increased when 1.00rag and 0. 10mg OVA was applied （P 〈 0.05）. The concentrations of OVA sIgE of 8-week male BN rats on the 28th day were significantly higher than that in the control group when 0. 10mg and 0.01 mg OVA was applied （P 〈 0.05 ）. And the concentrations of OVA sIgE of 8-week male BN rats on the 35th day were significantly higher than that in the control group when 1.00 mg OVA was applied （P 〈 0.05）. The concentrations of OVA sIgE of 4-week male BN rats on the 35th day were significantly higher than the control group when 1.00 mg OVA was applied （P 〈 0.05 ）.Conclusion Female rats were more sensitive than male rats to different doses of OVA administered intraperitoneally; 8-week rats were more sensitive than 4-week rats; the better dose of OVA for sensitizing BN rats was 0. 10mg or 1.00rag. Therefore, an ideally sensitized animal model could be established by administering 8-week female BN rats with 0. 10 mg or 1.00mg OVA intraperitoneally in 35 days later.