抑癌基因p15低表达与苯血液毒性相关性研究

Correlation of the down-regulation of tumor suppressor gene p15 and hematotoxicity from benzene exposure

目的探索抑癌基因p15低表达与苯血液毒性的关系。方法应用荧光定量PCR方法,检测20名苯中毒工人和17名同期接苯但无苯中毒表现的同工种对照以及19名健康对照外周血抑癌基因p15基因表达水平。结果与对照组比较,p15 mRNA表达水平在苯中毒组及接苯组均显著下调(P<0.01)。随着苯中毒严重程度的增加,p15表达水平显著下调,并有趋势显著性(P<0.01)。p15 mRNA表达水平与白细胞计数及中性粒细胞计数呈显著正相关(r=0.42,P=0.002;r=0.44,P=0.002)。结论抑癌基因p15在慢性苯中毒者中低表达。p15转录抑制与苯的血液毒性密切相关。

Objective To explore the correlation between the expression of tumor suppressor gene p15 and the hematotoxicity from benzene exposure. Methods Twenty cases of patients with chronic benzene poisoning （BP） , 17 benzene-exposed workers engaged in the same job with the patients but without BP and 19 matched unexposed controls from Tianjin were recruited. The expression of tumor suppressor gene p15 mRNA was detected by qRT-PCR. Results Comparing with the unexposed controls, the expression levels of p15 mRNA were significantly down-regulated in BP patients （P 〈 0.01） and also in benzene-exposed workers （P 〈 0.01 ）. The expression of p15 was down-regulated with the increased severity of BP （P 〈 0.01 ）. The expression of p15 mRNA is positively correlated with white blood cell counts and neutrophil counts （r = 0.42, P = 0. 002,r = 0.44, P = 0. 002, respectively）. Conclusion The expression of p15 mRNA is significantly down-regulated in BP patients. The transcriptional repression of p15 is associated with benzene hematotoxieity.