鲜地黄中梓醇及水苏糖的闪式提取工艺优选

Optimization of Flash-Type Extraction Process for Catalpol and Stachyose from Fresh Rehmannia glutinosa

目的:优选鲜地黄中梓醇及水苏糖的提取工艺。方法:以梓醇和水苏糖提取率为指标,采用HPLC进行含量测定,比较不同提取方法所得梓醇及水苏糖含量,采用L9(34)正交试验优选提取工艺条件。结果:与传统方法相比,闪式提取法效率更高,其最佳提取工艺为6倍量20%乙醇闪式提取3次,每次1 min。结论:闪式提取法是一种高效、快速提取鲜地黄中梓醇及水苏糖的方法。

Objective：To optimize extraction process of catalpol and stachyose from fresh Rehmannia glutinosa.Method：With extraction rate of catalpol and stachyose as indexes,the content of catalpol and stachyose were determined by HPLC.The content of catalpol and stachyose in different extraction methods were compared,and then extraction technology was optimized by L9（34） orthogonal test.Result：Compared traditional extraction method,flash-type extraction method had higher efficiency,optimal flash-type extraction technology was as follows： extracted 3 times with 6 times the amount of 20% ethanol,each time 1 min.Conclusion：Flash-type extraction process was an efficient and rapid method for extraction catalpol and stachyose from fresh R.glutinosa.