三虎痛风颗粒的提取工艺优选

Optimization of Extraction Process of Sanhu Tongfeng Granule

目的:优选三虎痛风颗粒的提取工艺。方法:采用正交试验,考察加水量、提取次数、提取时间3个因素对提取工艺的影响,以虎杖苷、大黄素含量及浸膏得率为指标,采用RP-HPLC测定虎杖苷、大黄素含量,烘干法测定浸膏得率。结果:最佳提取工艺为药材加10倍量水加热回流提取2次,每次1 h。结论:该工艺设计合理,稳定可行,适用于工业化生产。

Objective： To optimize extraction process of Sanhu Tongfeng granule. Method： Orthogonal design was used to investigate the amount of water, extraction times and extraction time with the content of polydatin, emodin and yield of extract as indexes. The content of polydatin and emodin were determined by RPHPLC, yield of extract was determined by drying method. Result： The best extraction conditions were as follows： refluxing extracted 2 times with 10-fold water, 1 h for each time. Conclusion： This optimized extraction process was reasonable, stable, feasible and applicable to industrial production.