HPLC同时检测丹参提取物中5种水溶性和脂溶性成分含量

Simultaneous Content Determination of Salvianolic acid A,Salvianolic acid B,Tanshinol,Lithospermum Acid,Tanshinone ⅡA in Crude Extract of Salvia miltiorrhiza Radix et Rhizoma by HPLC

目的:建立丹参提取物中丹酚酸A、丹酚酸B、紫草酸、丹参素及丹参酮ⅡA反相HPLC同步测定新方法。方法:选用Kromasil 100-5 C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水(含0.1%甲酸)为流动相梯度洗脱,流速1 mL.min-1,检测波长285 nm。结果:丹酚酸A、丹酚酸B、紫草酸、丹参素及丹参酮ⅡA分别在进样量范围内与峰面积线性关系良好,r>0.999 5,仪器精密度和稳定性RSD均<2%,成分的回收率在97%～103%(n=3)。测定了其5批丹参提取物含量。结论:该方法快速、准确、重复性好,可同时定量丹参的5种水溶性及脂溶性成分。

Objective： To establish a new RP-HPLC method for simultaneous determination of salvianolic acid A, salvianolic acid B, tanshinol, lithospermum acid, tanshinone ⅡA in crude extract of Radix Salvin Miltiorrhiza. Method： Analysis was carried out on a Kromasil 100-5 C18 （4.6 mm×250 mm,5μm） column with acetonitrile-pure water （added 0. 1% formic acid） as mobile phases in gradient elution. The flow rate was 1 mL·min^-1 and the UV detector was monitored at 285 nm. Result： All calibration curve showed good linear regression in test ranges （r 〉0. 999 5） ; and the overall recoveries were in the range of 97% -,103% （n =3） with RSD less than 3%. The overall RSD of precision test were less than 3%. Conclusion： The method is rapid, accurate, credible and repeatable, and can provide bases for the material basis and compatibility for Radix Salvin Miltiorrhiza.