火棘不同药用部位中槲皮素含量的比较

Comparison of the Contents of Quercitin in Various Medicinal Parts of Pyracantha fortuneana

目的:建立反相高效液相色谱法测定火棘中槲皮素含量的方法,并比较火棘不同部位中槲皮素的含量。方法:采用90%甲醇-25%盐酸(4∶1)为溶剂提取样品,利用反相高效液相色谱法测定火棘不同部位样品中槲皮素的含量。色谱柱Hypersil ODS(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸水溶液(40∶60),流速1.0 mL.min-1,检测波长360 nm,柱温25℃。结果:槲皮素在0.048 5～1.552 0μg线性关系良好,平均回收率为96.18%。结论:火棘不同部位中槲皮素的含量差异较大。

Objective： To established an RP-HPLC method for determination of quercetin in Pyracantha fortuneana and to compare quercetin contents in different medicinal parts. Method： 90% methanol-25% hydrochloride aqeous solution （4：1 ） was used to extract quercetin and Hypersil ODS （4.6 mm×250 mm,5μm） column was used to analyze quercetin content with methanol-0.1% phosphoric acid aqueous solution as mobile phases. Flow rate was 1.0 mL ~ rain-1. Detection wavelength was set at 360 nm. Result： The standard curve of quercetin was linear in the range of 0. 048 5-1. 552 0 p,g. The average recovery of quercetin was 98.44%. Conclusion： Quercetin contents in different medicinal parts of P. fortuneana was different.