润肤止痒乳对银屑病患者角质形成细胞增殖和VEGF分泌的影响

For patients with psoriasis skin itching milk cutin cell proliferation and formed the secretion of VEGF influence

目的:观察润肤止痒乳对银屑病患者角质形成细胞增殖和VEGF分泌的影响。方法:对银屑病患者表皮角质形成细胞进行原代培养,通过四甲基偶氮唑蓝微量酶反应比色法(MTT法)及酶联免疫吸附法(ELISA)体外观察润肤止痒乳对表皮角质形成细胞增殖及VEGF分泌的影响。结果:润肤止痒乳在0-485.5 mg/mL浓度范围内对角质形成细胞无毒副作用,在该浓度范围内有明显抑制角质形成细胞增殖的作用,其抑制作用与他扎罗汀比较,差异无统计学意义(P>0.05);且在该浓度范围内润肤止痒乳可减少角质形成细胞分泌VEGF的含量。结论:润肤止痒乳在一定浓度范围内可抑制角质形成细胞的增殖作用,且能减少VEGF的分泌。

Objective： To observe the itching for patients with psoriasis skin breast cell proliferation and VEGF cutin form the secretion of influence. Methods： The patients with psoriasis skin cutin form cells for the training, through the four methyl azo blue trace enzyme reaction was colorimetric method （MTT method） and ELISA method （ELISA） in vitro observation of embellish skin itching breast cell proliferation of epidermis cutin formation and the influence of VEGF secretion. Result： Skin itching milk in 0-485.5 mg/mL concentration range form cells to cutin no side effect, in this concentration worldwide significant inhibitory cutin form cell proliferation function, its inhibition and his firm LuoTing comparison, the difference was not statistically significant （P〉0.05）; And in this concentration range of embellish skin itching milk can reduce eutin form cells secrete VEGF content. Conclusion： Skin itching milk in a certain concentration range can inhibit cutin form cell proliferation effect, and can reduce the secretion of VEGF.