保肝颗粒中胡芦巴碱的含量测定

Determination of Content of Trigonelline in Baogan Granulas

目的:建立高效液相法测定保肝颗粒中胡芦巴碱含量的方法。方法:以胡芦巴碱的含量为检验指标,AgilentZORBA×NH2柱色谱(4.6 mm×250 mm,5μm),流动相水-甲醇-乙腈(20∶40∶40),流速1 mL.min-1,检测波长265 nm,进样量10μL,柱温40℃。结果:胡芦巴碱在进样量5～25μL(r=0.999 9),线性关系良好,平均回收率为98.3%(n=9),RSD%1.63%。结论:该方法操作简单、快速、专属性强,其含量测定可为保肝颗粒的质量控制提供科学依据。

Objective：To establish a HPLC method for determination of trigonelline in Baogan granulas.Method： The content of trigonelline was determined by HPLC.The Agilent ZORBA×NH2 column（4.6 mm×250 mm,5 μm） was used and mobile phase was composed of water-methanol-acetonitrile（20∶40∶40）.The flow rate of mobile phase was 1 mL·min-1 and detection wavelength was at 265 nm and the column temperature was maintained at 40 ℃.Result： The linear response range was from 5-25 μL（r=0.999 9）.The recovery rate was 98.3%（n=9）,RSD was 1.63%.Conclusion： HPLC method is simple,accurate and exclusive,which can provide the scientific basis for quality control of the Baogan granulas.