剑叶耳草水提物对急性肝损伤小鼠的保护作用

Protective Effect of Hedyotis lance Aqueous Extract on Acute Liver Injury in Mice

目的:研究剑叶耳草水提物(JYECA)对小鼠急性肝损伤的保护作用。方法:分别采用四氯化碳(CCl4)、D-半乳糖胺盐酸盐(D-GalN)ip建立小鼠急性肝损伤模型,用比色分析法测定小鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的活性;测定小鼠肝匀浆超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量;HE染色观察各组小鼠肝细胞损伤程度。结果:JYECA能显著降低因CCl4,D-GalN所致急性肝损伤小鼠血清ALT,AST的升高;对急性肝损伤小鼠肝匀浆SOD的活性有明显的升高作用及降低MDA的含量(P<0.01或P<0.05);能够减轻肝细胞损伤程度。结论:JYECA对CCl4、D-GalN所致小鼠急性肝损伤有保护作用,其机制可能与其抗氧自由基、抑制脂质过氧化作用有关。

Objective： To observe the protective effect of Hedyotis lance aqueous extract （JYECA） on acute liver injury in mice. Method： The models of carbon tetrachloride （CC14） and D-GaiN-induced acute liver injury in mice with the method of intraperitoneal injection were used to study the protective effects of JYECA. The colorimetric assay was used to measure the activity of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in blood serum, and the activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in liver homogenate were determined. Hematoxylin and eosin （HE） stain was used to examine the degree of hepatic injury. Result： JYECA could obviously reduce the activity of AST and ALT and the content of MDA in acute hepatic injury mice, and increase the activity of SOD （ P 〈 0.01 or P 〈 0.05）. JYECA could reduce the degree of hepatic injury. Conclusion： JYECA has protective effect on acute hepatic injuried mice. The mechanism may be related to attenuating free radical and inhibiting lipid peroxidation.