老年气虚血瘀型胸痹患者红细胞膜蛋白组成研究

The Composition of Erythrocyte Membrane Proteins of the Senile Coronary Heart Disease Patients with Qi Deficiency and Blood Stasis

目的:利用SDS-PAGE法研究老年气虚血瘀型胸痹患者红细胞膜蛋白组成的变化。方法:18例老年气虚血瘀型冠心病(胸痹)患者,以健康老年人(13例)和健康青年人(10例)为对照,分析红细胞膜蛋白组成变化。结果:与青年人比较,冠心病组带1蛋白、带3蛋白、带4.1蛋白、带4.2蛋白、带5蛋白、原肌球调节蛋白和带6蛋白百分含量均显著降低;且带1、带3、带4.1、原肌球调节蛋白百分含量均显著低于健康老人组。结论:气虚血瘀型冠心病心绞痛患者的膜蛋白组成发生变化,可能是导致抗氧化能力低下,膜流动性降低的原因之一。

Objective：To investigate the changes in the components of erythrocyte membrane proteins of the senile coronary heart disease patients with qi deficiency and blood stasis.Method： Eighteen coronary heart disease patients with Qi deficiency and blood stasis,thirteen healthy old people and ten healthy young people were the coronary patient group and healthy old people control group and healthy young people control group.The composition of erythrocyte membrane proteins was analysed by SDS-PAGE.Result：The percentage composition of band 1 protein,band 3 protein,band 4.1 protein,band 4.2 protein,band 5 protein,band 6 protein and tropomodulin of coronary patients were significantly lower than those of the healthy young people.The percentage composition of band 1 protein,band 3 protein and band 4.2 protein of the healthy old people were significantly higher than those of coronary patients.Conclusion： The mechanism of decreasing antioxidant ability and membrane fluidity may be related with the changes in composition of membrane protein.