离体肾灌流技术在药理毒理研究中的应用进展

Application of Method of Isolated Perfused Kidney into Pharmacological and Toxicological Studies

通过Pubmed和CNKI数据库查阅了近几年应用离体肾灌流(isolated perfused kidney,IPK)技术在药理毒理研究方面的文献,并进行了整理和分析。IPK技术的主要特点是一定时间内接近体内肾的外环境,通过不同时间点从输尿管和肾静脉流出的灌流液中采样,动态观察外源性物质进入肾脏所发生的变化。IPK技术可用于研究药物在肾脏的排泄过程、作用机制以及药物的联用等。此外,通过检测灌流液中蛋白质活性及含量的变化,结合肾组织病理学变化,还可用于研究药物对肾损伤特点、程度、机制等。该文对离体肾灌流的技术特点及其在药理毒理研究中的应用进行介绍,以供药物研究和临床应用参考。

The literature,in recent years,related to IPK in pharmacological and toxicological studies was compiled and analyzed.One of the peculiarities of its method is that the perfusion fluid may be pulled repeatedly from ureter and renal vein for dynamically detecting the change of xenobiotics and their metabolin in kidney in the short run.Therefore,the method may be applied to study the drug excretion,mechanism of action and drug combination etc.Furthermore,by determining the change of protein activity and content in perfusion fluid,combined with histopathological observation,the method may be used to study the characteristic,extent and mechanism of drug induced renal injury.This article summarized the characteristic of IPK and its application into the pharmacological and toxicological studies in order to provide reference for scientific research and clinical application of the drugs.