乙酰左旋肉碱预处理对低氧低糖诱导PC12细胞凋亡的影响

Effect of acetyl-L-carnitine preconditioning on PC12 cell apoptosis induced by oxygen-glucose deprivation

目的评价乙酰左旋肉碱预处理对低氧低糖诱导PC12细胞凋亡的影响。方法PC12细胞接种于96孔板中，采用随机数字表法，将细胞随机分为5组（n=6）：对照组（C组）、细胞损伤组（I组）和不同浓度乙酰左旋肉碱预处理组（A1-3组）。C组加入含葡萄糖4．5g／L的DMEM溶液孵育3h；I组加入含葡萄糖0．5g／L和Na2S2O4 3mmol／L的DMEM溶液孵育3h；A1-3组分别加入0．2、0．4和0．6mmol／L乙酰左旋肉碱孵育24h，然后加入含葡萄糖0．5g／L和Na2S2O4 3mmol／L的DMEM溶液孵育3h。采用MTT法检测细胞活力，采用TUNEL法检测细胞凋亡率，测定细胞SOD和ATP酶的活性和MDA含量。结果与C组比较，I组细胞活力降低，细胞凋亡率升高，SOD和ATP酶的活性降低，MDA含量增加（P〈0．05），A1-3组细胞活力、细胞凋亡率、SOD和ATP酶的活性和MDA含量差异无统计学意义（P〉0．05）；与I组比较，A1-3组细胞活力升高，细胞凋亡率降低，SOD和ATP酶的活性升高，MDA含量下降（P〈0．05）；A1-3组间细胞活力、细胞凋亡率、SOD和ATP酶的活性和MDA含量比较差异无统计学意义（P〉0．05）。结论乙酰左旋肉碱预处理可通过抑制细胞凋亡，减轻低氧低糖诱导PCI2细胞损伤。

Objective To investigate the effect of acetyl-L-carnitine （ALC） preconditioning on the PC12 cell apoptosis induced by oxygen-glucose deprivation. Methods PC12 cells were seeded in 96-well plates and randomly divided into 5 groups （ n = 6 each） ： control group （group C）, cell injury group （group I） and preconditioning with different concentrations of ALC groups （groups A1-3 ）. In group C, the cells were incubated with DMEM liquid culture medium containing glucose 0.5 g/L for 3 h. In groups I and A1-3 the ceils were incubated with DMEM liquid culture medium containing sodium hydrosulfite （Na2S2O4 ） 3 mmol/L and glucose 0.5 g/L for 3 h, and in addition the cells were pre-incubated with ALC 0.2, 0.4 and 0.6 mmol/L for 24 h in groups A1-3 respectively. Cell viability was evaluated by MTr assay, while the apoptosis in cells was detected using TUNEL. The activities of ATPase and SOD and MDA content were also detected. Results Oxygen-glucose deprivation significantly increased the number of apoptotic cells and the content of MDA, and decreased the cell viability and activities of SOD and ATPase in group I compared with group C （P 〈 0.05）. Preconditioning with ALC significantly increased the cell viability and the activities of SOD and ATPaes, and decreased the number of apoptotic cells and the content of MDA in groups A1-3 compared with group I （ P 〈 0.05）. Conclusion ALC preconditioning can attenuate PC12 cell injury induced by oxygen-glucose deprivation through inhibition of apoptosis in cells.