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PCNA、CXCR4在结直肠癌组织芯片中的表达及意义 被引量：2

The expression and significance of PCNA and CXCR4 protein in human colorectal carcinoma tissue in tissues-microarray
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摘要 目的探讨PCNA和CXCR4蛋白在结直肠癌组织芯片中的表达及其意义。方法在结直肠癌组织芯片上,应用免疫组化EnVision法检测PCNA和CXCR4表达水平,分析PCNA和CXCR4在结直肠癌的表达与患者淋巴结转移状态、肿瘤分化程度的关系及其相互关系。结果 PCNA在结直肠癌组织中的表达率为44.5%(57/128);CXCR4的表达率为43.8%(56/128),PCNA和CXCR4与患者淋巴结转移状态、肿瘤分化程度相关(P<0.05)、二者之间呈正相关(P<0.05)。结论同时检测结直肠癌组织中的PCNA和CXCR4蛋白的表达对判断肿瘤的恶性程度和估计预后有一定意义。 【Objective】 To evaluate the expression and significance of PCNA（Proliferative cell nuclear antigen） and CXCR4（CXC chemokin receptor 4） protein in human Colorectal carcinoma tissues in tissues-microarray.【Methods】 The expression levels of PCNA and CXCR4 protein were measured in human Colorectal carcinoma tissues by EnVision immunohistochemical method in tissues-microarray.To study the relationship between their expression and lymph node metastasis differentiations and their correlations.【Results】 The positive rates of PCNA and CXCR4 expression were 44.5%（57/128） and 43.8%（56/128） in colorectal carcinoma,respectively.The expression of PCND and CXCR4 was significantly correlated in colorectal carcinoma to lymph node metastasis and differentiations.PCNA have intimate relationship with CXCR4（P 〈0.05）.【Conclusion】 PCNA and CXCR4 may be good predictors of metastasis and prognostic evaluation for colorectal carcinoma patients.
作者 郭云娣 李明 何影娟
机构地区 苏州卫生职业技术学院苏州检验医学生物技术重点实验室 苏州市立医院 苏州九龙医院
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2012年第13期29-32,共4页 China Journal of Modern Medicine
关键词 结直肠肿瘤 核增殖抗原 趋化因子受体 组织芯片 colorectal carcinoma PCNA CXCR4 tissues-microarray
分类号 R735.3 [医药卫生—肿瘤]
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参考文献13

  • 1TAKANAMI I. Overexpression of CCR7 mRNA in nonsmall cell lung cancer: correlation with lymph node metastasis [J]. Int J Cancer, 2003, 105(2): 186-189.
  • 2KUSHLINSKII NE, ORINOVSKII MB, GUREVICH LE, et al. Expression of biomolecular markers (Ki-67, PCNA, Bcl-2, BAX, BclX, VEGF) in breast tumors[J]. Bull Exp Biol Med, 2004, 137 (2): 182-185.
  • 3苏丽萍,张进平,徐焕宾,陈晋,王缨,储以微,熊思东.CXCR4在肺癌转移中的作用及其机制研究[J].中华医学杂志,2005,85(17):1190-1194. 被引量：19
  • 4KOLLMAR O, RUPERTUS K, SCHEUER C, et al. Stromal cell derived factor-1 promotes cell migration and tumor growth ofvcolorectal metastasis[J]. Neoplasia, 2007, 9(10): 862-870.
  • 5张阳德,王苏阳,马琪,赵明钢.结肠癌肝转移的分子机制研究进展[J].中国现代医学杂志,2007,17(16):1986-1990. 被引量：9
  • 6ANDRE F, CABIOGLU N, ASSI H, et al. Expression of chemokine receptors predicts the site of metastatic relapse in patients with axillary node positive primary breast[J]. Ann Oncol, 2006, 17(6): 945-951.
  • 7LIANG Z, YOON Y, VOTAW J, et al. Silencing of CXCR4 blocks bleast cancer metastasis [J]. Cancer Res, 2005, 65 (3): 967-971.
  • 8LAPTEVA N, YANG AG, SANDERS DE. CXCR4 knockdown by small interfering RNA abrogates breast tumor growth in vivo[J].Cancer Gene Ther, 2005, 12(1): 84-89.
  • 9OTTAIANO A, FRANCO R, AIELLO TALAMANCA A, et al. Overexpression of both CXC chemokine receptor 4 and vascular endothelial growth factor proteins predicts early distant relapse in stage II-III colorectal cancer patients[J]. Clin Cancer Res, 2006, 12(9): 2795-2803.
  • 10WU YG, JIN M, XU HB, et al. Clinicopathologic Significance of HIF-1 a, CXCR4 and VEGF Expression in Colon Cancer[J]. Clin Dev Immunol(Published online), 2010, 2010: 537-531.

二级参考文献17

  • 1Feng Y, Broder CC, Kennedy PE, et al. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science, 1996, 272:872-877.
  • 2Muller A, Homey B, Soto H, et al. Involvement of chemokine receptors in breast cancer metastasis. Nature, 2001, 410:50-56.
  • 3Dunussi-Joannopoulos K, Zuberek K, Runyon K, et al. Efficacious immunomodulatory activity of the chemokine stromal cell-derived factor 1 (SDF-1):local secretion of SDF-1 at the tumor site serves as T-cell chemoattractant and mediates T-cell-dependent antitumor responses. Blood, 2002, 100:1551-1558.
  • 4Lu XL, Wang JX, Li XH, et al. Spontaneous metastasis of clonal cell subpopulation of human lung large cell carcinoma after subcutaneous inoculation in nude mice. Chin J Oncol, 1989, 11:3-7.
  • 5He C, He P, Liu LP, et al. Analysis of expressions of components in the plasminogen activator system in high- and low-metastatic human lung cancer cells. J Cancer Res Clin Oncol, 2001, 127:180-186.
  • 6Inoue K, Slaton JW, Eve BY, et al. Interleukin 8 expression regulated tumorgenicity and metastases in androgen-independent prostate cancer. Clin Can Res, 2000, 6:2104-2119.
  • 7Olson TS, Ley K. Chemokines and chemokine receptors in leukocyte trafficking. Am J Physiol Regulatory Integrative Comp Physiol, 2002, 283:R7-R28.
  • 8Freije JM, Balbin M, Pendas, et al. Matrix metalloproteinases and tumor progression. Adv Exp Med Biol, 2003, 532:91-107.
  • 9Wang N, Yu SH, Liener IE, et al. Characterization of high and low metastatic clones derived from a methycholanthrene induced murine fibrosarcoma. Cancer Res, 1982, 42:1046-1051.
  • 10Peled A, Kollet O, Ponomaryov T, et al. The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34+ cells:role in transendothelial /stromal migration and engraftment of NOD/SCID mice. Blood, 2000, 95:3289-3296.

共引文献26

同被引文献24

  • 1魏梅,梁立治,张昌卿,熊樱,张颖,沈扬,李锦清.宫颈腺癌细胞CXCR4/CXCL12过表达与淋巴结转移和慢性炎症的关系[J].癌症,2007,26(3):298-302. 被引量：16
  • 2PAULINO F.Carcinomam of the gall bladder and the extrahep- atic bile ducts[J].Rev Bras Cir,1953,25(1):740-753.
  • 3OLNES MJ,ERLICH R.A review and update on cholangiocarci- noma[J].Oncology,2004,66(3):167-179.
  • 4CARRIAGA MT,HENSON DE.Liver,gallblad der,extrahepatic bile ducts,and pancreas[J].Cancer,1995,75(1):171-190.
  • 5MULLER A,HOMEY B,SOTO H,et al.Involvement of chemokine receptors in breast cancer metastasis[J].Nature,2001,410(6824):50-56.
  • 6GULENG B,TATEISHI K,OHTA M,et al.Blockade of the stroma cell-derived factor-1/CXCR4 axis attenuate in vivo tumor growth by inhibiting angiogenesis in a vascular endothelialgrowth factor-independent mannei[J].Cancer Res,2005,65(13):5864-5871.
  • 7CHINNI SR,SIVALOGAN S,DONG Z,et al.CXCL12/CXCR4 signaling activates Akt-1 and MMP-9 expression in prostate cancer cells:the role of bone microenvironment-associated CX- CL12[J].Prostate,2006,66(1):32-48.
  • 8TERADA R,YAMAMOTO K,HAKODA T,et al.Stromal cell-derived factor-1 from biliary epithelial cells recruits CX- CR4-positive cells:implications for inflammatory liver diseases[J].Lab Invest,2003,83(5):665-672.
  • 9LAPIDOT T,KOLLET 0.The essential roles of the chemokine SDF-1 and its receptor CXCR4 in human stem cell homing and repopulation of transplanted immune-deficient NOD/SCID and NOD/SCID/B2m (null)mice[J].Leukemia,2002,16(10):1992-2003.
  • 10张春勇,余斌,陈克嶙,袁雪波,郭荣富.L-精氨酸对脂多糖诱导免疫应激大鼠血清生化指标和肝脏的影响[J].营养学报,2011,33(4):344-348. 被引量：4

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中国现代医学杂志
2012年 第13期

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