家蚕绵茧突变品系中部丝腺的差异蛋白组学分析

Differential Proteomics Analysis of Middle Silk Gland in Silkworm Strain with Flossy Cocoon Mutation

为了获取与家蚕绵茧突变形成相关蛋白质的基础信息,采用蛋白质双向电泳技术对结茧性状具有明显差异的正常茧、绵茧、丝胶茧3个家蚕品系5龄期幼虫中部丝腺不同区段的蛋白质进行双向电泳(2-DE)分析,图谱中的蛋白点主要集中在分子质量14～70 kD、等电点(pI)4～9的区域。采用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)技术对部分表达量差异显著的蛋白点进行鉴定,获得35种可信的蛋白质,绵茧突变品系与正常茧、丝胶茧品系相比表达量有显著差异的蛋白质包括参与能量代谢、蛋白质合成等生物学过程的功能蛋白质,其中丝氨酸蛋白酶抑制剂16(serpin16)和丝氨酸蛋白酶抑制剂18(serpin18)可能与家蚕绵茧突变形成相关。

To acquire basic information about proteins associated with flossy cocoon mutation in silkworm （Bombyx mori）, two-dimensional electrophoresis （2-DE） was carried out to analyze proteins from various sections of silk gland of the 5th instar larvae of 3 silkworm strains with different cocooning characters （i. e., normal cocoon, flossy cocoon and sericin cocoon）. The protein spots on the 2-DE map were mainly distributed in an area with molecular weight of 14 to 70 kD and pl of 4 to 9. Matrix-assisted laser desorption ionization and time of flight mass spectrometry （MALDI-TOF-MS） was applied for identifying protein spots with significant expressional difference and 35 protein spots were identified with reliability. Compared to silkworm strains spinning normal and sericin cocoons, the flossy mutant cocoon strain had protein spots of significant expressional difference that were involved in biological processes such as energy metabolism and protein synthesis. Among them, serine protease inhibitor 16 （serpin16） and serine protease inhibitor （serpin18） were found to associate with the formation of flossy cocoon mutation in silkworm.