摘要
改良的MTT比色法用10％SDS-5％异丁醇-0.01mol/L HCl溶解CTLL_2细胞内的甲?,得到稳定的甲?溶液,其0D_(570)值能保持几十小时基本不变,便于比色分析。测定重组白细胞介素2的结果与~3H-TdR掺入法大致相同,两者的相关系数为0.991(p<0.01)。
In the improved colorimetry for recombinant interleukin-2 (γIL-2), the formazan in mitochondria of CTLL_2 cells is dissolved in 10% SDS-5%isobutanol-0.01 mol/L HCl to offer a more stable purple solution with OD_(570)value hardly changing at least a few days. The correlation coefficient betweenthe results of this method and ~3H-TdR incorporation method is 0.991 (p<0.01).
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1990年第3期133-135,共3页
Chinese Journal of Pharmaceuticals
关键词
白细胞介素
MTT比色法
生物检定
recombinant interleukin 2
MTT colorimetry
CTLL_2 cell
