虎榛子愈伤组织对土生空团菌菌丝生长的影响

Influence of Callus of Ostryopsis davidiana on the Mycelium Growth of Cenococcum geophilum Fr.

新研究表明植物不能形成菌根的障碍是缺失一种植物形成菌根的信号物质,这种信号物质可以诱导菌根真菌菌丝的生长和分枝,为下一步研究植物与外生菌根真菌互作的生化机制和互作过程中的信号传导途径提供理论基础,建立虎榛子愈伤组织与其外生菌根真菌的共培养体系,在纯培养条件下采用干重法,测定虎榛子愈伤组织、愈伤组织匀浆液和愈伤组织分泌物对2株土生空团菌[Cenococcum geophilum Fr.(Cg)]菌株生长的影响。结果表明,供试Cg菌株与宿主的愈伤组织共同培养可以明显地促进菌丝的萌发和生长,而愈伤组织匀浆液和愈伤组织分泌物也可促进菌丝体早期生长,其中共培养法培养促生作用最强,尤为突出,2菌株单菌落的生物量分别达到63.5mg和45.2mg。初步研究表明,虎榛子愈伤组织与菌根菌共培养可以明显促进菌丝生长,证明虎榛子愈伤组织细胞在生长的过程中会分泌一类生化物质对外生菌根真菌菌丝的生长有促进作用,但究竟有哪些物质在起作用,是进一步研究的内容。

The latest research showed the obstacle of mycorrhizae formation was lack of some signal material that could induce mycorrhizal fungi mycelial to grow and branch. The co-cultures of callus of Ostryopsis davidiana and its mycorrhizal fungi were established for improving mycorrhizal fungi cultivation methods. And the secondary metabolites of the co-culture were studied. Using the dry weight method, the influence of the O. davidiana callus and homogenate and secretion of callus on the mycelium growth of Cenococcum geophilum Fr. （Cg） was investigated in vitro. The results showed that, the two strains which co-cultured with the host callus could obviously promote the fungal hyphae germination and growth. At the same time, homogenate and secretion of callus could also facilitate early mycelia growth. While the most effective method was the co-cultures, and the single colony dry maximum biomass of each Cg strain reached to 63.5 mg and 45.2 mg. The research of preliminary got a good cultural method of ectomycorrhizal fungi mycelium which could evidently upgrade the growth of the hyphae. The O. davidiana callus cells surely secreted some biochemical substances that promoted the growth and branch of hyphae in the progress of the growth.