细胞因子和一氧化氮在大鼠感染性脑水肿模型的时相变化

Time-course of cytokines and nitric oxide content in brain tissue of infectious brain edema induced by Pertussis Bacilli in rats

目的 了解白细胞介素 - 1(IL- 1)、肿瘤坏死因子 (TNF)及一氧化氮 (NO)在大鼠感染性脑水肿 (感脑 )时的变化及它们与感脑的关系。方法 百日咳菌液诱发大鼠感脑后 ,EL ISA法测大鼠脑组织匀浆中的 IL- 1β、TNFα的含量 ,Griess法测 NO含量。结果 注菌液后 2 40 m in大鼠脑组织匀浆中的 IL- 1β(384.1± 37.7pg/g)、TNFα(76 2 .2± 84.9pg/g)明显增高 ,与 C组、NS组及 PB5 min、30 min、6 0 m in、12 0 min组比较差异有非常显著性 (P<0 .0 1)。 NO注菌后 12 0 m in(94.4±11.6 nm ol/g)明显增多 ,2 40 m in(10 6 .0± 13.8nmol/g)达高峰 ,与 C组、NS组及 PB5 min、30 min、6 0 m in钟组比较差异有非常显著性 (P<0 .0 1)。结果 IL- 1β、TNFα和 NO与感脑关系密切 ,IL- 1β、TNFα和

Objective To understand time course of cytokines and nitric oxide(NO) content in brain tissue of infectious brain edema induced by Pertussis Bacilli in rats and relationship between those and infectious brain edema. Methods ELISA and Griess methods were used to measure the contents of IL 1 β、TNF α and NO in brain tissue in vivo after infectious brain edema by pertussis bacilli in rats. Results Contents of IL 1 β(384.1±37.7pg/g) and TNF α(762.2±84.9pg/g)were significantly increased 240min in PB group than in NS groups,C group and in other PB groups ( P <0.01).The concentration of NO was significantly increase 120min in PB group(94.4± 11.6 nmol/g)and in 240min PB group(106.0±13.8nmol/g)than in NS groups,C group and the other PB groups( P <0.01). Conclusion IL 1 β,TNF α,NO are closely related to infectious brain edema by Pertussis Bacilli,causing greater damage to infectious brain injury if developed in large amount.