PCR反向膜杂交技术检测乙型肝炎病毒

Detection of hepatitis B virus by PCR-reverse cross-blot hybridization method

目的探讨PCR反向膜杂交技术在乙型肝炎病毒 (HBVDNA)检测中的应用。方法用PCR反向膜杂交技术定性检测 10 0例血清标本中的HBVDNA ,结果与常规PCR法和酶联免疫吸附测定 (ELISA)方法比较。结果 10 0例血清标本 ,本法阳性率为 5 3 0 % ,常规PCR法为 47 0 %。HBsAg(+)和HBeAg(+)与HBVDNA有很好的相关性。结论该技术能快速、敏感和高度特异地对PCR产物进行鉴定 ,亦可检测HBV的真实感染和复制情况 ,有助于乙型肝炎的临床诊断。

Objective To evaluate the value of detecting the HBV DNA with PCR reverse cross blot hybridization assay.Methods 100 serum samples from hepatitis B patients were analyzed by PCR reverse cross blot hybridization.Results The hepatitis B virus DNA positive rate was 53% by the assay, but routin PCR was 47%.Between the HBsAg(+) with HBeAg(+),HBV DNA were close correlation.Conclusion PCR reverse cross blot hybridization was a rapid, sensitive, specific method to detect PCR product, and to help the clinical diagnosis of hepatitis B.