摘要
目的了解实验动物的感染情况 ,为更好地科学饲养、管理实验动物 ,提高实验动物质量奠定基础。方法采用Dot-ELISA法、肺印片姬氏染色法及改良抗酸染色法对 30只Wistar大鼠、10 0只昆明鼠及 12 0只家兔进行检查[1] 。结果大鼠、小鼠及家兔弓形虫的阳性感染率分别为 6 7%、5 %及 2 0 8%;卡氏肺孢子虫的阳性感染率分别为 2 0 %、2 %及 0 %;受检动物的粪便均未查到隐孢子虫卵囊。结论从实验的结果来看 ,我院实验动物中心提供的实验动物尚未达到贵州省制定的一级动物标准 ,表明我院实验动物中心在管理、饲养动物方面存在一定的不足。
Objective In order to understand condition of infective and improve the quality of experimental animals, infection of opportunity pathogenic parasites was investigated in some experimental animals.Methods 30 wistar rats, 100 mice of Kunming and 120 rabbits were examined by Dot ELISA, Giemsa’s stain and modified acid fast staining.Results The rat, mice and rabbits positive rate of Toxoplasma gonidii were 6 7%,5.0% and 20.8% , the pneumocystis carinii were 20%,2% and 0% respectively. The oocysts of cryptosporidium was not found in all fecal specimens. Conclusion the feeding and administration of experimental animals of Guiyang Medical College should be improved immediately
出处
《贵州医药》
CAS
2000年第2期73-74,共2页
Guizhou Medical Journal
关键词
机会致病原虫
姬氏染色
寄生虫感染
实验动物
Opportunity pathogenic parasite Dot-ELISA Giemsa’s stain Modified acid fast staining