海州常山(Clerodendrum trichotomum)提取液对凤眼莲(Eichhornia crassipes)生长的抑制作用

Inhibitory effects of harlequin glory-bower(Clerodendrum trichotomum) extract on growth of water hyacinth(Eichhornia crassipes)

研究海州常山(Clerodendrum trichotomum)的2种提取液对入侵性水生杂草凤眼莲(Eichhornia crassipes)叶片的抑制作用,并通过测定凤眼莲叶片的叶绿素含量、过氧化氢酶活性和丙二醛含量分析其抑制机制。结果表明:与空白组和对照组相比,喷施海州常山提取液的凤眼莲健康叶片比例低于1%(P<0.01),死亡叶片比例高于85%(P<0.01),凤眼莲叶片的叶绿素含量显著降低(P<0.05),过氧化氢酶活性显著升高(P<0.05),丙二醛含量极显著增加(P<0.01)。由于海州常山提取液作用于凤眼莲叶片,造成叶片的叶绿素含量降低,活性氧积累,生物膜系统受到损伤,从而导致凤眼莲叶片干枯腐烂甚至衰亡。说明海州常山提取液对凤眼莲的生长具有明显的抑制作用,为生物防治凤眼莲提供了一种新途径。

The inhibitory effects of harlequin glory-bower （Clerodendrurn trichotomurn） water extract on the growth of water hyacinth （Eichhornia crassipes）, an invasive aquatic weed which had resulted in enormous ecological and economic consequences in China, were explored and its inhibition mechanism was analyzed by measuring chlorophyll content, catalase activity and malondialdehyde content of water hyacinth leaves. The results showed that the healthy leaf proportion （less than 1%） of water hyacinth which was sprayed the extract was significantly lower than that of the blank group and control group （P〈0.01）, and the dead leaf proportion （more than 85%） of water hyacinth was significantly higher than theirs （P〈0.01）, and the chlorophyll content of leaves from treated groups was decreased （P〈 0.05）, and the catalase activity was enhanced （P〈0.05）, and the malondialdehyde content was significantly increased （P 〈 O. 01）. The extract of harlequin glory-bower acted on leaves of water hyacinth, so that the chlorophyll content was decreased, and the reactive oxygen was accumulated to damage biofilm system, resulting in leaves dry rot and decay. In conclusion, the extract of harlequin glory-bower inhibits effectively the growth of water hyacinth, which provids a new biological approach to control water hyacinth.