宝兴鼠尾草的有效成分提取及含量测定

Extraction and determination of active ingredients from Salvia paohsingensis

采用超声提取法、回流提取法及索氏提取法对宝兴鼠尾草有效成分的提取效果进行比较,并采用高效液相色谱法(HPLC)对所提取成分含量进行测定,并与栽培丹参的相应成分进行比较。结果表明:对于样品中的脂溶性成分,超声提取法以低温、高效的特点明显优于其他2种方法;而对于水溶性成分,索氏提取4h的效果更佳;对其含量分析表明,宝兴鼠尾草的脂溶性成分含量较高,其中丹参酮IIA的含量达到《中国药典》规定,但水溶性成分含量偏低。说明宝兴鼠尾草具有一定的药用价值,但能否作为丹参代用品有待进一步研究。

Ultrasonic extraction, alcohol reflux, and Soxhlet extraction all stood to be tested on a comparative basis for effect in active ingredients of Salvia paohsingensis extraction. The high performance liquid chromatography （HPLC） was applied for assay of extracted constituents, and they were compared with corresponding constituents in Salvia miltiorrhiza Bunge. The main results were as follows： with respect for the fat-soluble constituents, ultrasonic extraction featured with low temperature and high efficiency was better than other two methods, while the 4-hour Soxhlet extraction was better when considered the water-soluble constituents. The assay by HPLC showed that the fat-soluble constituents of S. paohsingensis were relatively high and the content of tanshinone IIA reached the level stipulated in Chinese Pharmacopoeia, nevertheless, the water-soluble constituents were relatively low. It is suggested that S. paohsingensis possesses certain medicinal value, however, whether it can be used as a substitute of S. rniltiorrhiza needs further research.