妙峰山玫瑰嫁接苗与自根苗生理生化指标比较

A Comparative Study on Physiological and Biochemical Indexes of Grafted and Non-Grafted Saplings of Miaofeng Mountain Rose

以日本无刺蔷薇(A)、美国多花无刺蔷薇(B)、美国M1号(C)为砧木嫁接妙峰山玫瑰(D),通过大棚盆栽对比试验研究妙峰山玫瑰嫁接苗与自根苗的光合特性和生理生化指标。结果表明:嫁接苗与自根苗的净光合速率(Pn)日变化规律相似,均呈单峰曲线,峰值出现在11:00—14:00,其中B+D的Pn最高,D最低;由综合最大净光合速率、光补偿点(LCP)、光饱和点(LSP)、表观量子效率(AQY)、水分利用效率(WUE)等指标可以看出,4种苗木的光合性能,以B+D最优,A+D最弱;C+D叶片光合色素各项指标的测定值最低,D最高;嫁接苗的保护酶活性略高于自根苗,而膜脂过氧化产物丙二醛含量是自根苗较高。

Taking different cultivars of rose as root stocks to be grafted with Miaofeng Mountain rose, a compar- ative experiment study on the photosynthetic and physio-chemical indexes between the grafted and self-rooted sap- lings of Miaofeng Mountain rose was conducted through potted experiment in the greenhouse. The results showed that the diurnal variation of P, of the saplings with different rootstocks was similar to that of the self-rooted seedlings with one peak curve, and the peak occurred between 11 am and 2 pm of the day time. Among the combinations, ＇ B ＋ D＇ showed the highest Pn value and the self-rooted seedlings had the lowest P, value. Based on the compre- hensive analyses on the indexes of the maximum photosynthetic rate （Pn）, light compensation point （LCP）, light saturation point （LSP）, apparent quantum yield （AQY） and water use efficiency （WUE）, the combination ＇B ＋ D＇ showed the best photosynthetic properties while the combination ＇ A ＋ D＇ was the last. Regarding the indexes of photosynthetic pigment content, the results also revealed that the combination ＇ C ＋ D ＇ had the lowest values whereas the self-rooted saplings had the highest values. The activity of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） in the leaves of the grafted saplings were slightly higher than those of the self-rooted seedlings, whereas the MDA content of the grafted saplings was lower.