HPLC法测定36%咪鲜·丙环唑悬浮剂中有效成分和2,4,6-三氯苯酚

Determination of Active Ingredients and 2,4,6-Trichlorophenol in Prochloraz+Propiconazole 36% SC by HPLC

建立了36%咪鲜·丙环唑悬浮剂中有效成分咪鲜胺、丙环唑以及相关杂质2,4,6-三氯苯酚的高效液相色谱定量分析方法,采用甲醇–乙腈–水(pH=3,磷酸调节)体系为流动相,150mm×4.6 mm Kromasil C18(5 m)不锈钢柱,紫外检测器(225 nm)。结果表明,本方法线性相关系数为0.999 9(有效成分)、0.998 7(杂质),标准偏差均小于0.10,变异系数均小于3.8%,平均回收率在99.8%～102.4%之间。

It was to establish a method for separation and determination of active ingredients and related impurity（2,4,6-trichlorophenol） in prochloraz ＋ propiconazole 36% SC by HPLC.The 150 mm×4.6 mm Kromasil C18（5 μm） stainless steel HPLC column and UV detector（detected at 225 nm） were used,with the mobile phase of methanol-acetonitrile-phsophoric acid solution（pH=3.0） at a flow rate of 1.0 mL/min.The results showed that the linear correlation coefficients were 0.9999（active ingredients） and 0.9987（impurity）,the coefficients of variation were less than 3.8%,and the average recoveries were between 99.8% and 102.4%.