HPLC法测定地西泮和艾司唑仑的血药浓度

Determination of diazepam and estazolam in human plasma by HPLC

目的:建立HPLC法同时测定地西泮和艾司唑仑血药浓度。方法:以UltrasphereODS5μm4.6mm×25cm色谱柱为分离柱,流动相:甲醇乙腈水(40∶20∶40);检测波长为254nm,以外标法峰面积定量。结果:地西泮和艾司唑仑的血浓度分别在0.214～2.568μg/ml,0.258～3.096μg/ml范围内与峰面积呈良好的线性关系,日内及日间差均小于10%,平均回收率分别为98.37%,99.19%。结论:本法可用于临床血药浓度测定。

OBJECTIVE :ToestablishaHPLCmethodforthedeterminationofdiazepamandestazolaminhuman plasma .METHOD :AUltrasphere ODScolum ( 5 μm ,4 .0mm× 2 5cm )wasusedwithamobile phaseofmethanol acetontrile water( 4 0∶ 2 0∶ 4 0 ) .Thedetectionwarelenythwas 2 5 4nm .RESULTS:Thelinearrangeofdiazepamand estazolamwereof 0 .2 1 4～ 2 .5 6 μg/ml,0 .2 5 8～ 3.0 96 μg/mlrespectively .Therelativestandarddeviationsforwithin day andbetween daysassayswerealllessthan 1 0 % .Therelativerecoveriesofdiazepamandestazolamwere 98.37%and 99. 1 9%respectively .CONCLUSION :Themethodissuitableforclinicalmonitorofdiazepamandestazolaminhumanplasma .