摘要
目的探讨肾虚对大鼠恐惧记忆形成的影响以及补肾方药的调节作用。方法以4月龄SD青年大鼠为对照组,复制自然衰老与"房室不节"两种大鼠肾虚模型,采用条件刺激(conditioned stimulus,CS)+非条件刺激(unconditioned stimulus,US)联合刺激以及CS+US非联合刺激训练大鼠。肾虚各组大鼠给予0.06g/kg淫羊藿总黄酮和2.025g/kg龟鹿二仙膏灌胃,对各组大鼠进行僵住行为测试。结果 CS+US联合刺激能显著提高青年大鼠、肾虚大鼠平均僵住时间百分比(P<0.05);肾虚大鼠僵住时间百分比明显增加(P<0.05),补肾方药能显著降低肾虚大鼠僵住时间百分比(P<0.05)。结论肾虚能够增强由CS+US联合刺激产生的条件性恐惧记忆,补肾方药能够抑制肾虚大鼠恐惧记忆的形成。
Objective To observe the effects of kidney-deficiency and kidney-tonifying prescriptions on the formation of fear memory in rats. Methods Four-month old Sprague-Dawley （SD） rats were used as controls.Two kinds of kidney-deficiency rat models induced by naturally aging and sexual exhaustion were employed. Rats were trained by conditioned stimulus （CS） and unconditioned stimulus （US） jointly or singly. The kidney-deficien- cy rats were treated with 2. 025 g/kg Guilu Erxian Gao （a patent traditional Chinese extract） and 0.06 g/kg total flavonoids of Epimedium, respectively. Average freezing time percentage （freezing%） was examined in rats of each group. Results CS combined with US could significantly enhance freezing%in young rats and kidney-deficiency rats （P〈0.05）. Freezing% was significantly increased in kidney-deficiency rats （P〈0.05）. Kidney-tonifying pre- scriptions could significantly decrease freezing% in kidney-deficiency rats. Cenclusien Kidney-deficiency could en hance conditioned fear memory caused by combined stimulus of CS and US. Kidney-tonifying prescriptions could inhibit the formation of fear memory of kidney-deficiency rats.
出处
《安徽中医学院学报》
CAS
2012年第3期48-52,共5页
Journal of Anhui Traditional Chinese Medical College
基金
国家自然科学基金(30873207)
关键词
条件性恐惧
肾虚
补肾
conditioned fear
kidney-deficiency
kidney-tonifying
