IBDV VP2蛋白P22表位多肽的原核表达及初步鉴定

Prokaryotic Expression and Identification of P22 Antigen Peptide on VP2 Protein of Infectious Bursal Disease Virus

为了进一步了解传染性法氏囊病毒(IBDV)VP2蛋白P22多肽抗原表位的功能,根据IBDVVP2蛋白的三维结构,设计合成编码P22多肽的基因序列,通过大肠杆菌密码子优化,并将基因序列P22串联二次合成并转化大肠杆菌BL21(DE3)菌株。用IPTG诱导P22基因的表达。表达纯化后的P22多肽经SDS-PAGE电泳和Western-blot分析及传染性法氏囊病毒快速检测试纸条检测。结果表明,P22表位多肽的分子质量约为16kD,并能被His单抗识别,用试纸条检测纯化的P22多肽,呈阳性反应结果。提示IBDV VP2蛋白P22多肽能与IBDV单抗特异性反应。

In order to study the function of P22 peptide on the VP2 protein of infectious bursal disease virus（IBDV）,the sequence of P22 peptide was designed according to three dimensional structures of VP2 on IBDV.P22 peptide was synthesized and expressed in E.coli BL21（DE） strain.After purification and renaturation,P22 peptide was analyzed by SDS-PAGE,Western-blot and IBDV rapid diagnostic strip.The results showed that the molecular weight of P22 peptide is about 16 kD and can be detected by anti-His monoclonal antibody.IBDV rapid diagnostic strip can interact with P22 peptide,indicating that anti-IBDV monoclonal antibody can detect P22 peptide specifically.