灵芝多糖对小鼠腹腔巨噬细胞活性氧自由基的影响

Effect of Ganoderma lucidum polysaccharides on oxygen free radicals in murine peritoneal macrophages

为进一步探讨灵芝多糖 ( GLP)免疫调节作用机理 ,采用激光扫描共聚焦显微镜技术 ,动态监测灵芝多糖均一体 GLB7对小鼠腹腔巨噬细胞 ( M )活性氧自由基含量的影响 .以 GLB7( 2 0 mg· L-1)刺激 M ,发现探针 DCHF- DA的荧光强度明显下降 ,与静息水平相比 ,平均下降 ( 36± 6) % ( n=6,P<0 .0 5) ;同时还能拮抗 PMA引起的 M 呼吸爆发 ,荧光强度下降幅度为 ( 1 9± 4) % ( n=6,P<0 .0 5) .结果证明 :GLB7能减少 M 内活性氧自由基的生成 ,具有清除活性氧的作用 ,这也是 GLB7产生免疫增强和抗衰老作用的重要途径 .

To investigate the mechanism of immunomodulation of Ganoderma polysaccharides, laser scanning confocal microscope was performed to indicate the effect of GLB 7 on oxygen free radicals in murine peritoneal macrophages. The results shown that GLB 7 could scavenge oxygen free radicals in murine peritoneal macrophages. It decreased the production of oxygen free radicals in murine peritoneal macrophages, the average percentage of decrease in the production of oxygen free radicals was about (36±6)%(n=6, P<0.05), and GLB 7(20 mg·L -1 ) antagonized the respiratory burst induced by PMA (5 nmol·L -1 ) in murine peritoneal macrophages(n=6, P<0.05). These observations suggest that GLB 7 decreased production of oxygen free radicals in murine peritoneal macrophages play an important role in the anti aging effect of Ganoderma polysaccharides.