摘要
目的:用免疫组化法观察大肠癌移植瘤诱导型一氧化氮合酶(iNOS)的表达。方法:以大肠癌裸鼠移植瘤为动物模型,将青春型双歧杆菌注射于裸鼠腹腔。结果:显示双歧杆菌注射组大肠癌移植瘤iNOS的表达率、表达强度和阳性细胞数量均显著高于肿瘤对照组(P<0.01)。结论:青春型双歧杆菌能增强大肠癌移植瘤iNOS的蛋白表达水平。它的表达可能介导了双歧杆菌诱导大肠癌移植瘤细胞的凋亡。
Depending on the animal model of colorectal carcinoma transplantation tumors of nude mice,the expression of inducible nitric oxide synthase (iNOS) of colorectal carcinoma transplantation tumors was detected by employing immunohistochemical staining, when bifidobacteria adolescence were injected into nude mice peritoneally. The results showed that the expression rate、 expression density and the number of positive cells of iNOS oncoproteins of colorectal carcinoma transplantation tumors in the bifidobacterium injection group was significantly higher when compared with the tumor control group (P<0 01). It was indicated that bifidobacteria adolescence could elevate the expression of iNOS might mediated the apoptosis of colorectal carcinoma transplantation tumors induced by bifidobacteria.
出处
《中国微生态学杂志》
CAS
CSCD
2000年第1期8-9,共2页
Chinese Journal of Microecology
关键词
双歧杆菌
大肠癌
诱导型
一氧化氮合酶
bifidobacterium
colorectal carcinoma
inducible nitric oxide synthase.
