当归多糖对大鼠脑缺血再灌注损伤的保护作用

Protective effects of angelica polysaccharide against focal cerebral ischemia and reperfusion injury in rats

目的观察当归多糖对大鼠脑缺血再灌注损伤和神经功能的影响。方法将40只大鼠随机分为假手术组、模型组及当归多糖低剂量组、当归多糖高剂量组,每组10只。分组后即开始腹腔注射不同剂量的当归多糖氯化钠注射液,每天1次,连续5 d。假手术组和模型组使用生理盐水。于第5天腹腔注射1 h后建立脑缺血再灌注模型,各组动物缺血3 h后再灌注24 h取材。其间观察大鼠的神经功能行为的变化,检测脑组织的超氧化物歧化酶(SOD)、谷胱甘肽(GSH)及谷胱甘肽过氧化物酶(GSH-PX)活性与丙二醛(MDA)的含量。结果相对于模型组,当归多糖处理组大鼠的神经功能损害明显减轻,脑组织的SOD、GSH及GSH-PX活性明显增高,而脑组织的MDA含量明显减少,而且具有明显的剂量依赖性。结论当归多糖能够减轻脑细胞缺血再灌注损伤,机制与清除自由基的作用有关。

Objective It is to observe the effects of angelica polysaccharide（APS） on focal cerebral ischemia reperfusion injury and nervous function in rats. Methods Fourty male Wistar rats were randomly divided into four groups （ten in every group） ：sham -operated group, model group, low and high dosage of APS treatment groups. The animals in treatment group were given different dosage of APS sodium chloride once every day by intraperitoneal injecting for five days. Sham - operated group and model group both were injected by sodium chloride. Rats model were established by intraluminal method of middle cerebral artery occlusion（MCAO） after intraperitoneal injecting for one hour on the 5th day. After ischemia for 3 hours, all an- imals were reperfused for 24 hours and were killed. The rats＇ nerve function behaviour was observed and activities of SOD, GSH, GSH- PX and MDA in cerebral tissue were determined. Results Compared with that in model group, nerve function behaviour was obviously relieved, the activities of SOD, GSH and GSH -PX were elevated, the content of MDA was decreased significantly, which depended evidently on the dosage of angelica polysaccharide. Conclusion Angelica polysaccharide can alleviate the cerebral ischemia and reperfusion injury, the mechanism of which depends on eliminating oxygen free radical.