荧光原位杂交技术在研究人精子染色体中的应用

Application of Fluorescence In Situ Hybridization in Chromosomal Analysis of Human Sperm

荧光原位杂交(FISH)技术以其简便、快捷、高敏感度及高特异度的优点,可一次检测成千上万的精子染色体而被广泛应用,并逐渐成为精子染色体检测的主要方法。许多研究用FISH技术检测男性的精子染色体发现,有毒物接触史、肿瘤患者放化疗后、染色体平衡易位患者及不育男性的非整倍体率较正常男性明显增加。特别是随着卵母细胞胞浆内单精子注射(ICSI)的应用,FISH在检测不育男性精子染色体中的作用更加明显。综述近年FISH在研究精子染色体非整倍体方面的进展。

Fluorescence in-situ hybridization（FISH） characterized by its simple,rapid,high sensitivity and high specificity has been the preferred method of chromosomal analysis of human sperm. FISH can detect thousands of sperm chromosomes in a batch of analysis. By FISH, it was found that the frequency of aneuploidy in those people contacting with poison, under chemo-treatment, or those people being chromosomal balanced translocation carrier and infertile men, was significantly increased than that in normal men. Especially with the development of intra-cytoplasmic injection （ICSI）,application of FISH in detecting sperm chromosomes of infertile men was significantly increased. We hereby reviewed the application of FISH technology in detecting aneuploidy of sperm chromosomes in recent years.