八角茴香中莽草酸提取工艺的优化

Optimization of Extraction Conditions of Shikimic Acid from Illicium Verum Hook.f Via Response Surface Methology

以水为溶剂,利用响应面法对八角茴香中莽草酸的提取工艺进行优化。在单因素试验的基础上,根据中心组合设计原理,采用三因素三水平的响应面分析法,依据回归分析确定最优提取工艺条件。选取提取时间、提取温度和料液比作为影响因子进行优化,结果表明,莽草酸的最佳工艺条件为提取时间3.0 h,料液比1∶22(g/mL),提取温度83℃,提取3次。在此条件下,一次提取率最高为8.779%。

Response surface analysis methodology（RSM） was used to optimize the extraction process of shikimic acid from illicium verum Hook.f with water as solvent.Based on single-factor experiments,the optimum conditions for the extraction of Shikimic Acid were obtained via Box-Benhnken center-united experiment design.Three independent variables,namely extracting time,solvent-solid ratio and extracting temperature,were selected as affecting factors during extraction.The results show that the optimum extraction conditions for shikimic acid were as follows： extraction time of 3.0 h,the ratio of material to solvent of 1∶22（g/mL）,extraction temperature of 83 ℃ for three times.In this condition,the yield of Shikimic Acid was up to 8.779 % at once.