一株耐高温乳酸菌的发酵条件优化

The Fermentation Medium Optimization of Lactic Acid at High Temperature

选用乳酸菌发酵生产L-乳酸,利用SAS软件的Plackett-Burman设计和Box-Benhnken设计优化了发酵培养基,以期提高L-乳酸的产量。最终确定最优培养基为(g/L),乙酸钠1.69、蛋白胨6.33、酵母膏6.63、葡萄糖30、吐温80 1、玉米浆10、柠檬酸二铵2、KH2PO40.28、MnSO4.7H2O 0.2、MgSO4.7H2O 0.2、CaCO310。在上述条件下,发酵乳酸菌的L-乳酸产量为12.533 g/L,比优化前提高74.07%。

The optimal fermentation medium of L-lactic acid production was studied by Plackett-Burman design and Box-Benhnken design of SAS system.The optimal fermentation medium was（g/L）,sodium acetate 1.69,peptone 6.33,yeast extract 6.63,glucose 30,tween 80 1,corn slurry 10,diammonium citrate 2,KH2PO4 0.28,MnSO4·7H2O 0.2,MgSO4·7H2O 0.2,CaCO3 10.Under the optimum medium,the yield of L-lactic acid was 12.533 g/L,with a increase of 74.07 %.