小柴胡汤联合环磷酰胺对腹水瘤细胞株肝癌小鼠肿瘤间质血管生成影响随机平行对照研究

Experimental Study of XiaoChaiHu Decoction Combined with Cyclophosphamide on Tumor Interstitial in Rats of EAC Liver Cancer Mice

[目的]观察小柴胡汤联合环磷酰胺对腹水瘤细胞株(EAC)肝癌小鼠体重、肿瘤间质血管生成影响。[方法]使用随机平行对照方法,将40只昆明小鼠,采用完全随机化分组方法分为模型组、中药组、西药组、联合组,每组10只。各组皮下注射浓度为2.0×106/ml的EAC肝癌小鼠细胞株0.2ml/只造模,分别给予小柴胡汤、环磷酰胺、小柴胡汤+环磷酰胺灌胃。免疫组化法检测血管内皮生长因子(VEGF)表达、光镜下观察形态学改变,透射电镜观察肿瘤组织血管生成。[结果]中药组、西药组、联合组VEGF阳性表达,瘤组织内的VEGF蛋白表达阳性率和平均染色强度均低于模型组(P<0.01),联合组最明显。光镜下显示:中药组、西药组、联合组肿瘤间质毛细血管数量均减少,联合组最为明显,中药组次之。电镜下显示:中药组、联合组都可以破坏肿瘤组织内新生毛细血管结构,联合组破坏最严重。[结论]小柴胡汤联合环磷酰胺可降低EAC肝癌小鼠瘤组织中VEGF蛋白表达;、抑制肿瘤间质血管生长。

[ Objective ] Discuss the anti - cancer function and mechanism of small entrapement soup and western chemotherapeutics cyclophosphamide on EAC mice affected liver cancer. [Methods] 40 mice from Kuming, hypodermic injection of 2.0 x 106/mL EAC cancer liver mice cell line 0.2mL/, treat it with en- trapement soup, cyclophosphamide, entrapement soup and cyclophosphamide separately, Immune group test the law endothelium growth factor express. The morphological changes were observed under the light micro- scope. Then find the situation of hepatumor cells with transmission electron microscope. [ Results ] lmmunohistochemistry showed, XiaoChaiHu group, CTX group, XiaoChaiHu ＋ CTX group VEGF po - sitive expression, XiaoChaiHu group, CTX group, XiaoChaiHu ＋ CTX group VEGF po - sitive expression rate and average strength in dyeing were lower than the control group, XiaoChaiHu ＋ CTX group to reduce the most obvious. Under light microscope, XiaoChaiHu group, CTX group, XiaoChaiHu ＋ CTX group can reduce the quantity of tumor stromal capillaries in newborn, XiaoChaiHu ＋ CTX group is the most obvious. XiaoChaiHu group is the second. Under electron microscope, XiaoChaiHu group, CTX group, XiaoChaiHu ＋ CTX group can damage the structure of tumor stromal capillaries in newborn , xeht ＋ CTX group to damage the most obvious. [ Conclusions ] XiaoChaiHu Decoction combined with Cyclophosphamide may reduce liver cancer in mice EAC tumor VEGF protein expression and restrain the grown of the Tumor interstitial vascularity.