Cry1Ab蛋白在拟环纹豹蛛体内的富集及对其体内酶活性的影响

Study on Bioaccumulation of CrylAb Protein and Activity of Enzymes in Pardosa pseudoannulata

用Cry1Ab蛋白处理的果蝇喂养拟环纹豹蛛Pardosa pseudoannulata，在第1d、3d、5d、7d、9d、11d用酶联免疫（ELISA）检测技术测定实验组和对照组拟环纹豹蛛体内Cry1Ab蛋白含量，运用紫外分光光度法检测拟环纹豹蛛体内超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）、乙酰胆碱酯酶（AChE）的活性，探讨拟环纹豹蛛体内Cw1Ab蛋白的富集作用与其体内3种酶活性的关系。结果表明，从第ld至第7dC^IAb蛋F1在拟环纹豹蛛体内均具富集作用（P〈0．05），到第7d的时候达到最高值，达极显著差异（p〈0．01），然后逐渐降低，但仍显著高于对照（p〈0．05）。SOD酶活性总体趋势是降低的，在第7d达到最低；用Cry1Ab蛋白处理拟环纹豹蛛后其体内的AChE和GSH—Px酶活性随饲养时间的延长而增加，并均高于对照组（p〈0．05）。该研究征明Cry1Ab蛋白在拟环纹豹蛛体内具有富集效应，并且其体内Cry1Ab蛋白对其体内GSH—Px、AChE具铂一定诱导作用，对SOD酶具有一定程度的抑制作用。

To investigate the relationship of CrylAb protein bioaccumlation and activities of three enzymes in the Pardosa pseudoannulata, we fed the spider with Drosophila melanogaster treated with CrylAb protein, then used enzyme-linked immunosorbent（ELISA） test to measure the CrylAb protein content of control and treated spider, and utilized UV spectrophotometry to determine activities of superoxide dismutase （SOD）, glutathione peroxidase（GSH-Px）, acetylcholinesterase（AChE） in 1 d, 3 d, 5 d, 7 d, 9 d, 11 d respectively. The results indicated that, CrylAb protein in the spider showed a effect of enrichment from 1^st d to 7^th d （p 〈0.05）, reached the peak in the 7^th day at a very significant difference （p 〈0.01）, and then gradually reduced, which was still significantly higher than control（p 〈0.05）. SOD activity grew lower in the overall trend, and reached the bottom at the 7^th day; activities of AChE and GSH-Px in the spider treated with the CrylAb protein increased with the extension of feeding time （p 〈0.05）. The study proved that CrylAb protein in the Pardosa pseudoannulata had a enrichment effect, and showed a induction to GSH-Px, ACHE, but a certain degree of inhibition to SOD enzyme.