大熊猫肠道抗生素耐药菌质粒的研究

Study on the plasmids of antibiotic-resistance bacteria in intestine of giant pandas

对分离自四川省卧龙保护区大熊猫肠道的6株大肠杆菌的抗生素抗性与质粒的关系进行了研究,结果显示:6株大肠杆菌中都存在不同大小的质粒DNA;采用SDS-高温法处理每株大肠杆菌后,有2类质粒在琼脂糖凝胶上的条带数减少,菌株的抗生素抗性水平也有所降低,有2株菌的某些抗生素抗性完全消失;将分离自6株大肠杆菌的质粒转化大肠杆菌JM109,获得1个氨苄青霉素抗性转化子.这些结果表明,大熊猫肠道中的大肠杆菌的抗生素抗性与它们含有的质粒有关.

The relationships of antibioticresistance bacteria in intestine of giant pandas and plasmids separated from Sichuan Wolong National Nature Reserve were analyzed. The results showed that 6 E.coli contained different sizes plasmid DNA. SDS and elevated growth temperature （43 ℃） were used to cure the plasmids. Of the 6 kinds of plasmid profiles, two plasmids were showed the disappearance of some plasmid bands. Regarding to the antibioticresistance level, it was found that the treatment of curing agents usually resulted in the decreased resistance level; even 2 lost some antibiotics resistance completely. These 6 kinds of plasmids were transformed into E.coli JM109 separately. One of them generated ampicillinresistant transformants. The results showed that resistance of antibioticresistance bacteria in intestine of giant pandas were related to plasmids.