门静脉高压性胃病小鼠模型的建立

Establishment of portal hypertensive gastropathy model in mice

目的:建立门静脉高压性胃病(PHG)小鼠模型;检测PHG小鼠胃黏膜细胞凋亡和活化Caspase-3表达情况。方法:分别采用部分缩窄门静脉联合左肾上腺静脉结扎(PPVL+LAVL)、腹腔注射四氯化碳建立C57BL/6小鼠门静脉高压模型(32只小鼠随机分为4组,包括假手术组、PPVL+LAVL组以及对照组、四氯化碳组),测量门静脉压力,进行肝胃组织苏木素-伊红染色、TUNEL标记检测染色计算凋亡指数、免疫组织化学检测活化Caspase-3表达情况,比较各组结果。结果:与假手术组相比,PPVL+LAVL组门静脉压力高[(9.5±0.8)mm Hg对比(5.9±0.4)mm Hg,P<0.01],凋亡指数高(9.2±0.9对比0.7±0.3,P<0.01),胃组织损害明显;与对照组相比,四氯化碳组肝组织呈中重度肝纤维化,门静脉压力高[(8.8±0.9)mm Hg对比(6.1±0.5)mm Hg,P<0.01],凋亡指数高(8.2±1.9对比1.9±0.6,P<0.01),胃组织出现损害。PPVL+LAVL组和四氯化碳组小鼠胃黏膜活化Caspase-3表达明显增多。结论:该研究成功建立两种小鼠PHG模型;PHG小鼠胃黏膜细胞凋亡增多,活化Caspase-3表达增多。

Objective： To establish the portal hypertensive gastropathy model in mice and to further investigate the apoptosis and the expression of cleaved Caspase-3 in gastric tissues of mice with portal hypertensive gastropathy. Methods： Thirty-two mice were randomly divided into 4 groups （partial portal vein ligation plus left adrenal vein ligation （PPVL ＋ LAVL） group, CCl4 group, sham operation group and control group）. Portal hypertension models were established by partial portal vein ligation plus left adrenal vein ligation （ PPVL ＋ LAVL）, and by intraperitoneal injection of carbon tetrachloride （ CCI4）, respectively. Portal pressures were measured. HE staining was applied pathological observation of hepatic and gastric tissues. Apoptosis index and cleavage Caspase-3 expression were assessed via TUNEL and immunohistochemistry, respectively. Results： Comparing with sham operation group, significantly higher portal pressure [ （9.5 ±0. 8） mm Hg vs （5.9±0. 4） mm Hg, P 〈0. 01 ], higher ap- optosis index （9.2 ± 0. 9 vs. 0. 7 - 0. 3, P 〈 0. 01 ）, and more prominent gastric mucosa injury were observed in PPVL ＋ LAVL group. Comparing with control group, moderate-severe hepatic fibrosis, significantly higher portal pressure [ （8.8±0.9） mm Hg vs （6.1 ±0. 5） mm Hg, P〈0.01], and higher apoptosis index （8.2 ±1.9 vs. 1.9±0. 6, P 〈0. 01） were revealed i.n CCI4 group with some gastric mucosa impairment. The expression of cleaved Caspase-3 was significantly increased in PPVL ＋ LAVL and CCI4 group. Conclusion： Two portal hypertensive gastropathy models in mice were established. Elevated apoptosis and expression of cleaved Caspase-3 are observed in portal hypertensive gastropathy mice.