萘胁迫下秋茄MnSOD基因和C4H基因的实时定量表达分析

Expression analysis of MnSOD gene and C4H gene in Kandelia candel under naphthalene stress

研究首次探讨了萘胁迫下红树植物秋茄不同组织C4H基因和MnSOD基因的表达特征。研究结果表明,两个基因在叶茎根中的表达量有明显的差异,C4H基因在叶茎中的表达量明显大于根,MnSOD基因的表达量为叶最大,茎次之,根最小。在5个不同浓度的萘胁迫下,C4H基因和MnSOD基因的表达普遍被诱导,转录水平都随着处理浓度的升高而增强,而且在叶组织中的表达量和萘处理浓度表现出明显的相关关系,相关系数分别达到0.846和0.902(p<0.05),而在根和茎中并不具有显著的相关关系。在萘胁迫下秋茄叶子是最敏感的部位,而C4H基因和MnSOD基因在叶子中的表达量则为指示萘胁迫强度的良好生物学指标。

The expression of C4H gene and MnSOD gene in leaf, stem and root ofKandelia candel responded to the naphthalene （NAP） was evaluated in this study. Real-time PCR analysis demonstrated that the regulation patterns in three tissues were different. The expression of C4H gene in leaf and stem was higher than that in root. The expression level of MnSOD was in the order of leaf 〉stem〉root. The expression level of C4H and MnSOD increased with the enhancement of NAP. A significantly positive correlation was also found between NAP and C4H expression in leaf, and the correlation coefficient was 0.846 and 0.902 （p〈0.05）, respectively. However, there was no obvious correlation between NAP and C4H expression in stem or root. The results indicated that the C4H and MnSOD gene regulation systems were the most sensitive in leaf, and the expression analysis indicated that they were good indicators for NAP stress.