三种鲫鱼品系同工酶比较研究

Comparative studies on isozymes of three strains of Carassius auratus

采用聚丙烯酰胺凝胶垂直板电泳技术,对彭泽鲫、银鲫D系以及野鲫三种鲫鱼品系的心、肝和肾脏组织的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、酯酶(EST)和超氧化物歧化酶(SOD)同工酶表型进行了比较研究。结果表明彭泽鲫乳酸脱氢酶同工酶比银鲫D系在肝组织多出二条酶带(LDH7’和LDH8’);超氧化物歧化酶在心和肾组织中分别多出一条酶带(SOD12’),表明彭泽鲫和银鲫已在生化水平产生明显的分化,推测它们可能起源于不同的地区,由不同的祖先,独立演化而形成。此外,彭泽鲫和银鲫D系的同工酶电泳图谱都包含野鲫的基本酶带,而彭泽鲫和野鲫的酯酶同工酶电泳图谱尤为相似,推测彭泽鲫和银鲫可能起源于野鲫,而彭泽鲫和野鲫的关系较近,银鲫和野鲫的关系较远。

The expressions patterns of five isozymes （LDH, MDH, ME, EST and SOD） were investigated in different tissues of Pengze crucian carp （Carassius auratus）, silver crucian carp D （C. auratus gibelio）, and wild crucian carp （C. auratus）, respectively, using polyacrylamide gel eleetrophoresis. Two bands （LDH7＇and LDH8＇） of LDH isozyme in liver tissue of Pengze crucian carp were more than those of silver crucian carp D. The main differences in SOD existed at heart and kidney, which showed one more band （SOD12＇） of Pengze crucian carp than those of silver crucian carp D. The results indicated that Pengze crucian carp is different from sliver crucian carp in biochemical level; they perhaps come from different region and have different ancestor. Meanwhile, the electrophoretogram of Pengze crucian carp and silver crucian carp all included the basal patterns of wild crucian carp. Specially, EST isozyme patterns between Pengze crucian carp and wild crncian carp were similar among the patterns of several isozyme. It is presumed that Pengze crucian carp and silver crncian carp were all originated from wild crucian carp. The relation between Pengze crucian carp and wild crucian carp is closer than that of silver crucian carp and wild crucian carp.