邻苯二甲酸酯联合暴露对杜氏盐藻生长的影响

Effect of combined exposure to dimethylphthalate and diethylphthalate on growth of Dunaliella salina

联合毒性效应是指两种或两种以上的化学物质同时或短时期内先后作用于机体所产生的综合毒性作用。论文探讨了典型环境激素邻苯二甲酸二甲酯(DMP)与邻苯二甲酸二乙酯(DEP)对杜氏盐藻(Dunaliella salina)的联合毒性效应。实验以96 h的单一毒性效应EC50为一个毒性单位(TU),将上述两种环境激素按照毒性单位比1:1、1:4和4:1的三种比例水平两两组合进行联合毒性试验,研究不同处理下,两种典型环境激素对杜氏盐藻生长的影响。结果表明:两种环境激素对杜氏盐藻生长均有抑制作用,且联合暴露较单一暴露对杜氏盐藻的细胞生长和光合作用抑制更强;DMP和DEP单一暴露对杜氏盐藻的96 h EC50分别为317.49 mg.L-1、和69.54 mg.L-1。两者在1:1、1:4和4:1三个比例水平上的联合毒性效应均表现为相加或协同效应,其中比例为1:1的协同效应最强。

Combined toxic effect may result from the concurrent exposure of multiple toxicants. In this study, the combined toxic effect of dimethylphthalate （DMP） and diethylphthalate （DEP） on growth of Dunaliella Salina was evaluated by taking the 96h EC50 as one toxic unit （TU）. The single toxic effect as well as combined toxic effect on the biomass, soluble protein content and photosynthetic pigment content ofD. salina was detected based on the different toxicity unit proportion of 1：1,1 ：4, and 4：1. The results showed that both DMP and DEP inhabited the growth ofD. Salina. Moreover, the toxic effect of simultaneous exposure was more significant than single exposure of the two chemicals. The values of96h ECs0 of DMP and DEP on D. salina were 317.49 mg.L-1 and 69.54 mg.L-1, respectively. Synergetic effect was observed for the toxicity of DMP and DEP in three toxicity unit proportions, which was more obvious in the proportion of 1：1.