姜黄素灌胃不同时间和剂量对小鼠胃肠蠕动的影响

Effects on gastrointestinal peristalsis of mice by curcumin gavage in different time and dose

【目的】探讨姜黄素作用不同时间和剂量对胃肠蠕动的影响,寻找姜黄素作用的最佳时间和剂量。【方法】昆明种小鼠,随机分组,每组8只。姜黄素[200mg/(kg.d)]预先灌胃分为1、5、10d和15d组;姜黄素灌胃10d,每公斤体重100、200、300、400mg组。采用阿托品腹腔注射减弱胃肠运动模型,用墨汁灌胃法分别测量各组小鼠胃残留率和小肠推进率。【结果】阿托品导致小鼠胃残留率增加,小肠推进率显著下降。姜黄素灌胃1和5d组,小鼠胃残留率和小肠推进率与阿托品组比均无明显变化,而姜黄素灌胃10和15d组,小鼠胃残留率显著下降,小肠推进率明显提高(P<0.01),10d和15d组间差异无统计学意义。100～400mg/kg姜黄素灌胃10d,对阿托品小鼠胃残留率和小肠推进率均有显著改善,各组间无明显差异。【结论】姜黄素100mg/(kg.d)、灌胃10d可以显著提高阿托品减弱的小鼠胃肠动力。

[Objective] To study the effects on promoting gastrointestinal peristalsis by curcumin lavage in different time and dose and to find out the optimal time and dose of curcumin. [Methods] Male mice were randomly divided into next groups：control group, atropine group and curcumin 1,5,10,15 day groups. Curcumin E200 mg/（kg ~ d）3 was adminis- trated by gastric lavage daily. In different concentration group, the mice were divided into 100,200,300 mg and 400 mg groups. Curcumin was administrated by gastric lavage daily for 10 days. The gastric residiual rate and the motional rate of small intestine were detected using the ink lavage method. [Results] In atropine group,the gastric residiual rate was in- creased and the motional rate of small intestine was decreased obviously. There were no significant improves in curcumin l days and 5 days groups. While in curcumin 10 days and 15 clays groups,the gastric residiual rate was decreased and the mo- tional rate of small intestine was increased significantly. There was no obvious difference in 10 days and 15 days groups. Cur- cumin （100~400 mg/kg） decreased gastric residiual rate and increased the motional rate of small intestine significantly. There was no different effects in different concentration of curcumin. [Conclusion] Curcumin E200 mg/（kg · d）, 10 days] can significantly improve the gastrointestinal peristalsis decreased by atropine in mice.